4Y72
Human CDK1/CyclinB1/CKS2 With Inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97957 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.166, 68.646, 166.771 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.086 | 0.778 |
Number of reflections | 34085 | |
<I/σ(I)> | 13.7 | 2.4 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.7 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.7 | 277 | Conditions around 0.1M MES/imidazole buffer (pH6.7), 6.5% MPD, 5% PEG4K, 10% PEG1K Protein at 10-12 mg/ml |