4Y6T

Structure of Tobacco streak virus coat protein dimer at 2.4 Angstroms resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-30
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 1 21 1
Unit cell lengths	103.040, 69.980, 103.440
Unit cell angles	90.00, 105.51, 90.00

Refinement procedure

Resolution	99.670 - 2.400
R-factor	0.2093
Rwork	0.207
R-free	0.24430
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.724
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	99.673	40.340	2.530
High resolution limit [Å]	2.400	7.590	2.400
Rmerge		0.034	0.692
Rmeas	0.101
Rpim	0.036	0.013	0.264
Total number of observations	422296	13312	61035
Number of reflections	54445
<I/σ(I)>	16.3	40.7	3.2
Completeness [%]	97.8	97.9	97.2
Redundancy	7.8	7.3	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	12% PEG 8000, 10% glycerol, 0.5M potassium chloride