4Y5S

Structure of FtmOx1 with a-Ketoglutarate as co-substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97625
Spacegroup name	P 1 21 1
Unit cell lengths	60.532, 45.659, 105.201
Unit cell angles	90.00, 99.98, 90.00

Refinement procedure

Resolution	36.126 - 2.543
R-factor	0.1785
Rwork	0.176
R-free	0.23400
RMSD bond length	0.017
RMSD bond angle	0.925
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.130	2.634
High resolution limit [Å]	2.543	2.543
Rmerge		0.691
Number of reflections	18963
<I/σ(I)>	10.529	1.5
Completeness [%]	99.9	99.35
Redundancy	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293.15	100 mM MES pH6.5, 50 mM CoCl2, and 2 M of ammonium sulfate