4Y59

Crystal structure of ALiS1-Streptavidin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-29
Detector	ADSC QUANTUM 315
Wavelength(s)	0.90
Spacegroup name	P 1 21 1
Unit cell lengths	57.843, 84.353, 46.156
Unit cell angles	90.00, 98.92, 90.00

Refinement procedure

Resolution	50.000 - 1.220
R-factor	0.12615
Rwork	0.125
R-free	0.15545
RMSD bond length	0.024
RMSD bond angle	2.343
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.240
High resolution limit [Å]	1.220	1.220
Rmerge	0.069	0.380
Number of reflections	129491
<I/σ(I)>	10.3	3.2
Completeness [%]	99.9	99.9
Redundancy	6.4	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1M Hepes-NaOH (pH7.0), 50%(v/v) MPD