4Y3K
Structure of Vaspin mutant E379S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-30 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.959, 151.353, 61.602 |
| Unit cell angles | 90.00, 98.43, 90.00 |
Refinement procedure
| Resolution | 48.370 - 2.200 |
| R-factor | 0.1878 |
| Rwork | 0.186 |
| R-free | 0.21930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4if8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS (November 11, 2013) |
| Data scaling software | XSCALE (November 11, 2013) |
| Phasing software | REFMAC (5.7.0029) |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.370 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.125 | 1.413 |
| Number of reflections | 60841 | |
| <I/σ(I)> | 7.2 | 0.9 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 2.5 M ammonium sulfate, 0.52 mM heparin oligosacchraide dp4, 0.1 M sodium citrate |
