4Y2O
Structure of CFA/I pili chaperone-major subunit complex (CfaA-CfaB)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 |
| Unit cell lengths | 129.192, 129.192, 73.271 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.464 - 2.419 |
| R-factor | 0.2368 |
| Rwork | 0.236 |
| R-free | 0.26230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f84 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.346 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.464 | 2.400 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.079 | 0.474 |
| Number of reflections | 18960 | |
| <I/σ(I)> | 20 | 1 |
| Completeness [%] | 96.4 | 72.2 |
| Redundancy | 7.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Na2HPO4-NaH2PO4 pH 6.6, 9% PEG8000 |
