4Y24
Complex of human Galectin-1 and TD-139
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97622 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.578, 58.242, 110.976 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.538 - 2.320 |
| R-factor | 0.2053 |
| Rwork | 0.200 |
| R-free | 0.24790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.770 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.5.3) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.400 |
| High resolution limit [Å] | 2.320 | 4.990 | 2.320 |
| Rmerge | 0.092 | 0.049 | 0.274 |
| Total number of observations | 87647 | ||
| Number of reflections | 12357 | ||
| <I/σ(I)> | 10.8 | ||
| Completeness [%] | 96.5 | 99.8 | 86.3 |
| Redundancy | 7.1 | 7 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Tris (pH 8.0), 0.2 M Li2SO4, 30% (w/v) PEG 3350 |
