4Y1L

Ubc9 Homodimer The Missing Link in Poly-SUMO Chain Formation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-04-10
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	63.230, 34.860, 114.510
Unit cell angles	90.00, 98.53, 90.00

Refinement procedure

Resolution	33.317 - 2.700
R-factor	0.2278
Rwork	0.225
R-free	0.27820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.169
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	33.320
High resolution limit [Å]	2.700
Number of reflections	13980
<I/σ(I)>	8.9
Completeness [%]	99.6
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	293	10% (w/v) PEG8000, 100 mM HEPES, pH 8.0, 8% ethylene glycol