4Y0V

Structure of an ADP ribosylation factor from Entamoeba histolytica HM-1:IMSS bound to Mg-GDP

Replaces:  3RD1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-18
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	123.540, 40.660, 78.950
Unit cell angles	90.00, 97.45, 90.00

Refinement procedure

Resolution	19.729 - 1.800
R-factor	0.2252
Rwork	0.223
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1r8s
RMSD bond length	0.007
RMSD bond angle	1.127
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1932))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.729		1.850
High resolution limit [Å]	1.800	8.050	1.800
Rmerge	0.060	0.022	0.431
Rmeas	0.069	0.026	0.486
Total number of observations	141065
Number of reflections	34091	406	2600
<I/σ(I)>	15.97	38.05	3.24
Completeness [%]	93.4	90	96.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	15% ethanol, 0.1M Tris, Cryoprotection 25% Ethylene glycol, pH 7.0