4Y0G
beta2 carbohydrate binding module (CBM) of AMP-activated protein kinase (AMPK)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-20 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.953700 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.973, 27.627, 78.882 |
| Unit cell angles | 90.00, 133.88, 90.00 |
Refinement procedure
| Resolution | 40.720 - 1.600 |
| R-factor | 0.15154 |
| Rwork | 0.150 |
| R-free | 0.18247 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.889 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.720 | 1.620 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.669 | |
| Number of reflections | 23221 | |
| <I/σ(I)> | 17.8 | 2.8 |
| Completeness [%] | 97.6 | 90.7 |
| Redundancy | 7.5 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 281.15 | 0.17 M ammonium sulphate, 15% v/v glycerol and 25.5 % w/v PEG4000 |
