4XZS
Crystal Structure of TRIAP1-MBP fusion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-22 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.390, 56.010, 100.280 |
| Unit cell angles | 90.00, 106.35, 90.00 |
Refinement procedure
| Resolution | 48.410 - 2.120 |
| R-factor | 0.19813 |
| Rwork | 0.196 |
| R-free | 0.24163 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hsj |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.470 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.410 | 2.180 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.093 | 0.585 |
| Number of reflections | 45307 | |
| <I/σ(I)> | 9.3 | 2.2 |
| Completeness [%] | 98.7 | 94 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 100 mM sodium acetate, 25% (w/v) PEG 4000, 18% (w/v) MPD, 200 mM ammonium sulphate |
