4XZQ

Nucleosome disassembly by RSC and SWI/SNF is enhanced by H3 acetylation near the nucleosome dyad axis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-02
Detector	NOIR-1
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	105.400, 109.570, 180.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.810 - 2.400
Rwork	0.256
R-free	0.29950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p3l
RMSD bond length	0.006
RMSD bond angle	1.017
Data reduction software	d*TREK (9.7L)
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.810	42.810	2.490
High resolution limit [Å]	2.400	5.170	2.400
Rmerge	0.079	0.052	0.418
Total number of observations	405916	31206	43709
Number of reflections	78406
<I/σ(I)>	9.4	20.1	2.8
Completeness [%]	95.0	86.7	99.9
Redundancy	5.07	3.91	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	292.15	potassium chloride, potassium cacodylate, manganese chloride