4XZC

The crystal structure of Kupe virus nucleoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-25
Detector	ADSC QUANTUM 270
Wavelength(s)	1.00
Spacegroup name	P 41 21 2
Unit cell lengths	73.981, 73.981, 423.978
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.059 - 2.601
R-factor	0.2153
Rwork	0.212
R-free	0.28040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3u3i
RMSD bond length	0.009
RMSD bond angle	1.150
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge		0.422
Number of reflections	37285
<I/σ(I)>	20.92	4.296
Completeness [%]	98.5	93.9
Redundancy	11.3	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	200mM sodium nitrate (PH 8.0), 5mM cobalt chloride, 8% (wt/vol) PEG 3350