4XXI

Crystal structure of the Bilin-binding domain of phycobilisome core-membrane linker ApcE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-01-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97092
Spacegroup name	P 2 21 21
Unit cell lengths	36.356, 80.552, 115.449
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.276 - 2.200
R-factor	0.189
Rwork	0.185
R-free	0.25130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xxk
RMSD bond length	0.014
RMSD bond angle	1.480
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.320
High resolution limit [Å]	2.200	2.200
Rmerge		0.215
Number of reflections	17930
<I/σ(I)>	25.4	9
Completeness [%]	99.9	99.6
Redundancy	8.5	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	285	0.1 HEPES, pH 7.0, 29% (w/v) PEG400, 0.2 M NaCl