4XXF
L-fuculose 1-phosphate aldolase from Glaciozyma antarctica PI12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 84.330, 84.330, 78.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.710 - 1.340 |
| R-factor | 0.1722 |
| Rwork | 0.171 |
| R-free | 0.18700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ocr |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.063 |
| Data reduction software | Coot |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.710 | 1.410 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmerge | 0.104 | |
| Number of reflections | 61957 | |
| <I/σ(I)> | 14.6 | 2 |
| Completeness [%] | 96.9 | 81.9 |
| Redundancy | 15.7 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | PEG 8000, magnesium chloride, Tris-HCl |
