4XWX
Crystal structure of the PTB domain of SHC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 64.910, 126.790, 50.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.980 - 1.870 |
| R-factor | 0.16887 |
| Rwork | 0.168 |
| R-free | 0.19096 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.644 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.980 | 1.970 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.060 | 0.823 |
| Number of reflections | 17581 | |
| <I/σ(I)> | 13.4 | 2.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277.15 | 0.8 M sodium/potassium tartrate, 0.1 M Hepes pH 7.5 |
