4XWT
Crystal structure of RNase J complexed with UMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2014-11-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.590, 87.760, 253.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.473 - 2.003 |
R-factor | 0.2019 |
Rwork | 0.200 |
R-free | 0.23520 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.121 |
Data reduction software | XSCALE |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.060 | |
High resolution limit [Å] | 2.000 | 8.960 | 2.000 |
Rmerge | 0.065 | 0.037 | 0.657 |
Rmeas | 0.072 | 0.041 | 0.716 |
Total number of observations | 588280 | ||
Number of reflections | 98253 | 602 | 7177 |
<I/σ(I)> | 16.09 | 31.16 | 3.25 |
Completeness [%] | 97.5 | 46.6 | 98.1 |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | PEG 4000, MnCl2, MES |