4XVT

Crystal structure of HIV-1 93TH057 coreE gp120 with antibody 45-VRC01.H01+07.O-863513/45-VRC01.L01+07.O-110653 (VRC07_1995)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	64.647, 67.841, 199.219
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.106 - 1.690
R-factor	0.1831
Rwork	0.181
R-free	0.22230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ngb
RMSD bond length	0.017
RMSD bond angle	1.823
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.110	1.760
High resolution limit [Å]	1.690	1.700
Rmerge	0.137
Number of reflections	97827
<I/σ(I)>	13	1.08
Completeness [%]	99.9	99.9
Redundancy	9.6	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	0.1 M Na Cacodylate pH 6.5, 12% PEG 8000, 0.16 M NaAcetate.