4XVJ

STRUCTURE OF THE HEPATITIS C VIRUS ENVELOPE GLYCOPROTEIN E2 ANTIGENIC 2 REGION 412-423 BOUND TO THE BROADLY NEUTRALIZING ANTIBODY HC33.1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-11-09
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.541
Spacegroup name	P 21 21 21
Unit cell lengths	36.085, 53.728, 107.238
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.000
R-factor	0.225
Rwork	0.225
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.060
High resolution limit [Å]	2.000	2.000
Rmerge	0.084	0.396
Number of reflections	14203
<I/σ(I)>	13.3	4.3
Completeness [%]	94.9	88.9
Redundancy	9.33	8.66

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	PEG 8000, MPD