4XU6

Crystal structure of cross-linked MvINS R77C trimer at 1.9A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	H 3
Unit cell lengths	85.596, 85.596, 126.120
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.071 - 1.898
R-factor	0.1895
Rwork	0.189
R-free	0.20320
RMSD bond length	0.007
RMSD bond angle	1.117
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	1.898
Number of reflections	26955
<I/σ(I)>	29.8
Completeness [%]	98.9
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	PEG 400