4XTT
Structural Studies of Potassium Transport Protein KtrA Regulator of Conductance of K+ (RCK) C domain in Complex with Cyclic Diadenosine Monophosphate (c-di-AMP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2014-10-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97934 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 84.976, 84.976, 85.575 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.347 - 2.708 |
R-factor | 0.2331 |
Rwork | 0.229 |
R-free | 0.27400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4j91 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.997 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 5.810 | 2.700 |
Rmerge | 0.145 | 0.057 | 0.641 |
Total number of observations | 101464 | ||
Number of reflections | 9002 | ||
<I/σ(I)> | 6.8 | ||
Completeness [%] | 99.8 | 98.5 | 100 |
Redundancy | 11.3 | 10.9 | 9.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100mM Bis-Tris pH 6.0, 24.5%(w/v) PEG6000, 250mM Lithium acetate |