4XTS
Salmonella typhimurium AhpC T43A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 |
| Unit cell lengths | 107.651, 109.454, 119.037 |
| Unit cell angles | 93.33, 110.02, 114.00 |
Refinement procedure
| Resolution | 63.511 - 2.704 |
| R-factor | 0.1927 |
| Rwork | 0.190 |
| R-free | 0.24460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ma9 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.655 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.500 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 118972 | |
| <I/σ(I)> | 5.2 | 1 |
| Completeness [%] | 96.3 | 92.9 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.2 M ammonium acetate, 25% polyethylene glycol 3350, and 0.1 M BIS-TRIS pH 6.5. |
