4XTR

Structure of Get3 bound to the transmembrane domain of Pep12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-10
Detector	PSI PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	102.870, 112.030, 153.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.950 - 2.050
R-factor	0.1886
Rwork	0.187
R-free	0.21610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2woj and 3pgf
RMSD bond length	0.003
RMSD bond angle	0.762
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1839))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.950	2.120
High resolution limit [Å]	2.050	2.050
Number of reflections	109335
<I/σ(I)>	11.7	2.1
Completeness [%]	98.0	90
Redundancy	3.8	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	2 mM ATP and 2 mM MgCl2 with 15% PEG 3350, 25 mM succinic acid pH 7.0