4XTN
Crystal structure of the light-driven sodium pump KR2 in the pentameric red form, pH 4.9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-21 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.969 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.690, 107.000, 240.800 |
| Unit cell angles | 90.00, 90.11, 90.00 |
Refinement procedure
| Resolution | 48.900 - 2.200 |
| R-factor | 0.1968 |
| Rwork | 0.195 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xtl |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.832 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.900 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.975 | |
| Number of reflections | 192342 | |
| <I/σ(I)> | 7 | 1.2 |
| Completeness [%] | 96.2 | 97.8 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | The crystals were grown using in meso crystallization technique |
