4XTM
Crystal structure of a recombinant Vatairea macrocarpa seed lectin complexed with GalNAc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.915 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 56.354, 86.111, 129.023 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.150 - 2.700 |
| R-factor | 0.207 |
| Rwork | 0.205 |
| R-free | 0.24400 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.396 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.2.7) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.150 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.111 | 0.394 |
| Number of reflections | 7221 | |
| <I/σ(I)> | 6.4 | 2.3 |
| Completeness [%] | 81.7 | 84.8 |
| Redundancy | 3.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 293 |
