4XT1
Structure of a nanobody-bound viral GPCR bound to human chemokine CX3CL1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | I 4 |
| Unit cell lengths | 81.024, 81.024, 231.303 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.234 - 2.886 |
| R-factor | 0.2043 |
| Rwork | 0.199 |
| R-free | 0.24920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mbs 1f2l 3ona 4b41 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.605 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1839) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.930 |
| High resolution limit [Å] | 2.880 | 7.810 | 2.880 |
| Rmerge | 0.200 | 0.114 | |
| Rmeas | 0.214 | 0.122 | |
| Rpim | 0.084 | 0.041 | 0.524 |
| Total number of observations | 141947 | ||
| Number of reflections | 16648 | ||
| <I/σ(I)> | 5.2 | ||
| Completeness [%] | 98.7 | 99.5 | 74.8 |
| Redundancy | 8.5 | 8.5 | 6.8 |
| CC(1/2) | 0.995 | 0.304 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 6.3 | 293 | PEG 300, MES, sodium succinate, polypropylene glycol 400 |
