4XOW
Structure of rsGreen0.7 in the green-on-state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-11 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.758, 61.594, 105.703 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.110 - 1.250 |
| R-factor | 0.1416 |
| Rwork | 0.141 |
| R-free | 0.16490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.339 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.110 | 1.290 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.077 | 0.670 |
| Number of reflections | 109670 | |
| <I/σ(I)> | 9.7 | 1.9 |
| Completeness [%] | 98.8 | 99.6 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 289 | 0.1 M Tris pH 8.5, 25% PEG 3350 |
