4XN2
Crystal Structure of Met260Ala mutant of E. coli Aminopeptidase N in complex with L-Leucine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-02-22 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 120.277, 120.277, 169.813 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.180 - 2.110 |
R-factor | 0.12562 |
Rwork | 0.124 |
R-free | 0.16486 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hpo |
RMSD bond length | 0.020 |
RMSD bond angle | 1.892 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.190 |
High resolution limit [Å] | 2.110 | 2.110 |
Number of reflections | 81193 | |
<I/σ(I)> | 26.32 | 4.89 |
Completeness [%] | 99.1 | 94.8 |
Redundancy | 5.8 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Sodium malonate |