4XME
Crystal structure of nitrophorin 7 from Rhodnius prolixus at pH 7.8 complexed with NO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-20 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.887, 67.052, 39.082 |
| Unit cell angles | 90.00, 117.35, 90.00 |
Refinement procedure
| Resolution | 34.710 - 1.290 |
| R-factor | 0.1723 |
| Rwork | 0.171 |
| R-free | 0.20450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mvf |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.074 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 2011_08_24_1020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.710 | 1.320 |
| High resolution limit [Å] | 1.290 | 1.290 |
| Rmerge | 0.032 | 0.925 |
| Number of reflections | 83829 | |
| <I/σ(I)> | 9.5 | 1 |
| Completeness [%] | 94.0 | 84 |
| Redundancy | 1.7 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 25%(w/v) PEG 3350, 0.1 M bis-Tris propane pH 7.8, 0.25%(w/v) Gly-Gly, 0.25%(w/v) Gly-Gly-Gly, 0.25%(w/v) Gly- Gly-Gly-Gly, 0.25%(w/v) pentaglycine, 0.02 M HEPES sodium pH 6.8. |
