4XKO

N-terminal domain of Hsp90 from Dictyostelium discoideum in hexagonal form with PEG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-05
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97625
Spacegroup name	P 62 2 2
Unit cell lengths	158.190, 158.190, 46.110
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	51.780 - 2.455
R-factor	0.2185
Rwork	0.216
R-free	0.26590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xcj
RMSD bond length	0.008
RMSD bond angle	1.057
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.780	2.590
High resolution limit [Å]	2.455	2.455
Rmerge	0.072	0.741
Number of reflections	12933
<I/σ(I)>	24.2	4
Completeness [%]	100.0	100
Redundancy	14.2	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	290	0.1M Tris, Ammonium sulphate, PEG 200