4XK4
E. coli transcriptional regulator RUTR with dihydrouracil
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-06 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 1 |
Unit cell lengths | 50.892, 55.672, 85.242 |
Unit cell angles | 88.37, 88.80, 62.37 |
Refinement procedure
Resolution | 50.010 - 2.270 |
R-factor | 0.19566 |
Rwork | 0.194 |
R-free | 0.22539 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jyk |
RMSD bond length | 0.010 |
RMSD bond angle | 1.354 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.010 | 2.310 |
High resolution limit [Å] | 2.270 | 2.270 |
Rmerge | 0.056 | 0.550 |
Number of reflections | 37742 | |
<I/σ(I)> | 14.6 | 1.67 |
Completeness [%] | 97.5 | 91 |
Redundancy | 2.2 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 200 nl of 10 mg/ml protein in 10 mM HEPES pH 7.5, 500 mM NaCl, 5 mM dihydrouracil was mixed with 200 nl 50 mM Ammonium Slufate, 50 mM BIS-TRIS, pH 6.5, 30% (v/v) Pentaerythirtol ethoxylate and suspended over a reservoir of 1.5M NaCl |