4XJF
X-ray structure of Lysozyme B1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2014-02-03 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.92049 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.100, 79.100, 38.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.550 - 1.800 |
| R-factor | 0.1774 |
| Rwork | 0.176 |
| R-free | 0.20680 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.089 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 11797 | |
| <I/σ(I)> | 12.9 | 3.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 32.3 | 33.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 1M NaBr, 50-100 mM CH3COONa, pH4.5, 15-30% pEG 400 |
