4XIZ
Structure of a phospholipid trafficking complex with substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.022, 74.123, 87.912 |
Unit cell angles | 90.00, 95.14, 90.00 |
Refinement procedure
Resolution | 34.130 - 2.000 |
R-factor | 0.2018 |
Rwork | 0.196 |
R-free | 0.24000 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9-1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.116 | 0.082 | 0.375 |
Total number of observations | 150394 | ||
Number of reflections | 36740 | ||
<I/σ(I)> | 10.7 | ||
Completeness [%] | 99.4 | 96 | 99.9 |
Redundancy | 4.1 | 3.9 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 2%(v/v)Tacsimate pH 6.0, 0.1M Bis-Tris pH 6.5, 20%(w/v) PEG3350 |