4XIG
Crystal structure of bacterial alginate ABC transporter determined through humid air and glue-coating method
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.175, 133.412, 272.687 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.559 - 3.402 |
R-factor | 0.2383 |
Rwork | 0.236 |
R-free | 0.28950 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.820 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.460 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.536 | |
Number of reflections | 37120 | |
<I/σ(I)> | 12.7 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 7.2 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG3000, N-(2-acetamido)iminodiacetic acid, sodium chrolide, 3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxypropanesulfonate |