4XIF
Human OGT in complex with UDP-5S-GlcNAc and substrate peptide (keratin-7)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 275.138, 275.138, 143.136 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 3.200 |
| R-factor | 0.21593 |
| Rwork | 0.216 |
| R-free | 0.23893 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.356 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 3.200 |
| Number of reflections | 105151 |
| <I/σ(I)> | 1.7 |
| Completeness [%] | 99.0 |
| Redundancy | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.45 M potassium phosphate, 10 mM EDTA, 1% (w/v) xylitol |
