4XID

AntpHD with 15bp DNA duplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ DW
Temperature [K]	85
Detector technology	IMAGE PLATE
Collection date	2014-04-25
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	96.443, 96.443, 90.142
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.601 - 2.701
R-factor	0.2285
Rwork	0.224
R-free	0.27430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	9ant
RMSD bond length	0.005
RMSD bond angle	0.746
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX ((phenix.refine: dev_1810))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.750
High resolution limit [Å]	2.700	7.320	2.700
Rmerge	0.054	0.018
Total number of observations	94639
Number of reflections	12086
<I/σ(I)>	12.7
Completeness [%]	99.0	98.9	97.3
Redundancy	7.8	6.8	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	300	20 mM BTP, 10 mM NiCl, 5% MPD