4XHZ
Crystal Structure of Human Protocadherin-15 EC8-10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 157.929, 157.929, 142.898 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 136.770 - 2.803 |
| R-factor | 0.17145 |
| Rwork | 0.170 |
| R-free | 0.19954 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ubh 1edh 3q2w |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.247 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 136.770 | 2.876 |
| High resolution limit [Å] | 2.800 | 2.803 |
| Rmerge | 0.077 | 0.680 |
| Number of reflections | 26222 | |
| <I/σ(I)> | 24.95 | 4.09 |
| Completeness [%] | 99.4 | 99.68 |
| Redundancy | 7.8 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277.15 | 0.1M MES (pH6.5), 1.6M NaCl |
