4XHY
NADH:FMN oxidoreductase from Paracoccus denitrificans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-18 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.81 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 43.910, 43.910, 154.660 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 51.550 - 1.530 |
R-factor | 0.16 |
Rwork | 0.158 |
R-free | 0.20500 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.987 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 51.550 |
High resolution limit [Å] | 1.500 |
Number of reflections | 28207 |
<I/σ(I)> | 44.12 |
Completeness [%] | 98.2 |
Redundancy | 8.26 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 4000, ammonium sulfate, ammonium acetate, MES |