4XHW

Crystal structure of Timeless_PAB domain in SeMet-labelled form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97923
Spacegroup name	C 2 2 21
Unit cell lengths	66.597, 100.083, 149.581
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.930 - 2.850
R-factor	0.1954
Rwork	0.193
R-free	0.25340
RMSD bond length	0.011
RMSD bond angle	1.397
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	24.930
High resolution limit [Å]	2.850
Number of reflections	12777
<I/σ(I)>	27.1
Completeness [%]	100.0
Redundancy	14.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	298	25% PEG 3350, 200mM sodium acetate