4XH8
Crystal Structure of E112A/D230A Mutant of Stationary Phase Survival Protein (SurE) from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 92.340, 92.340, 150.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.140 - 3.560 |
| R-factor | 0.26368 |
| Rwork | 0.262 |
| R-free | 0.30423 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v4n |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.923 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.300 | 3.900 |
| High resolution limit [Å] | 3.560 | 3.560 |
| Rmerge | 0.560 | |
| Number of reflections | 6585 | |
| <I/σ(I)> | 4.9 | 1.8 |
| Completeness [%] | 81.2 | 82.4 |
| Redundancy | 4.3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.08M Magnesium chloride, 0.1M HEPES (pH 7.5), 15 % polyacrylic acid sodium salt |
