4XH7
Crystal structure of MUPP1 PDZ4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0093 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 51.142, 52.681, 96.844 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.650 |
| R-factor | 0.1742 |
| Rwork | 0.173 |
| R-free | 0.20380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3jxt |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.372 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.773 | |
| Number of reflections | 16041 | |
| <I/σ(I)> | 48.2 | 3.7 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 7.2 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 2%(v/v) polyethylene glycol 400, 0.1 M imidazole, 24%(w/v) polyethylene glycol monoethyl ether 5000 |
