4XH0
Structure of C. glabrata Hrr25 bound to ADP (SO4 condition)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 71.788, 76.974, 84.277 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.499 - 1.990 |
| R-factor | 0.1676 |
| Rwork | 0.166 |
| R-free | 0.20040 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.250 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.800 | 2.100 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.095 | 0.910 |
| Number of reflections | 31839 | |
| <I/σ(I)> | 11.9 | 2.4 |
| Completeness [%] | 98.0 | 98.6 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M Bis-Tris pH 6.5, 0.2 M lithium sulfate, 19% PEG 3350, and 5 mM TCEP |
