4XGL

Structure of the nuclease subunit of human mitochondrial RNase P (MRPP3) at 1.8A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	P 43
Unit cell lengths	99.250, 99.250, 50.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.625 - 1.800
R-factor	0.1899
Rwork	0.189
R-free	0.22760
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.920
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		1.850
High resolution limit [Å]	1.800	8.050	1.800
Rmerge	0.092	0.039	2.270
Rmeas	0.097	0.041	2.386
Total number of observations	488725
Number of reflections	46217	565	3393
<I/σ(I)>	12.89	49.79	0.89
Completeness [%]	99.9	99.6	99.8
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	potassium citrate, PEG3350, magnesium chloride