4XGJ
Crystal structure of a domain of unknown function (DUF1537) from Pectobacterium atrosepticum (ECA3761), Target EFI-511609, APO structure, domain swapped dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-12 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 153.772, 47.844, 63.792 |
| Unit cell angles | 90.00, 106.69, 90.00 |
Refinement procedure
| Resolution | 41.530 - 1.900 |
| R-factor | 0.158 |
| Rwork | 0.156 |
| R-free | 0.19200 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.295 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.650 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.062 | 0.650 |
| Number of reflections | 35312 | |
| <I/σ(I)> | 18.9 | 2.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.4 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | Protein (46.8 mg/ml, 10 mM HEPES pH 7.5, 5 mM DTT); Reservoir (MCSG4 (F4); 0.2 M Magnesium Chloride, 0.1 M Tris pH 8.5, 16 %(w/v) PEG 4000); Cryoprotection (80% Reservoir, 20% glycerol) |
