4XGC
Crystal structure of the eukaryotic origin recognition complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-06-27 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.0332, 1.71083 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 145.545, 258.983, 257.001 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.799 - 3.500 |
R-factor | 0.2253 |
Rwork | 0.224 |
R-free | 0.25810 |
Structure solution method | SAD |
Starting model (for MR) | SAD SOLUTION |
RMSD bond length | 0.000 |
RMSD bond angle | 0.700 |
Data scaling software | Aimless |
Phasing software | SHELX |
Refinement software | PHENIX ((phenix.refine: dev_1760)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.800 | 3.590 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.214 | 2.181 |
Number of reflections | 61418 | |
<I/σ(I)> | 7.7 | 1.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 8.4 | 8.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | PIPES (pH 7.5), magnesium chloride, ammonium acetate, PEG 20000 |