4XG9
Crystal structure of an inhibitor-bound Syk
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-10 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.068, 42.094, 87.899 |
| Unit cell angles | 99.90, 90.42, 100.66 |
Refinement procedure
| Resolution | 50.000 - 2.910 |
| R-factor | 0.21008 |
| Rwork | 0.208 |
| R-free | 0.24104 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xg2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.328 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 11885 | |
| <I/σ(I)> | 11.16 | |
| Completeness [%] | 98.6 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 278 | 10~20%(v/v) PEG3350, 100 mM Tris-HCl |
