4XFP

Crystal Structure of Highly Active Mutant of Bacillus sp. TB-90 Urate Oxidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-27
Detector	RAYONIX MX-225
Wavelength(s)	1.0000
Spacegroup name	P 21 21 2
Unit cell lengths	134.135, 145.195, 71.141
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.818 - 1.660
R-factor	0.1571
Rwork	0.156
R-free	0.18410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wlv
RMSD bond length	0.006
RMSD bond angle	1.030
Data reduction software	SCALEPACK
Data scaling software	HKL-2000
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.9-1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.680
High resolution limit [Å]	1.650	4.470	1.650
Rmerge	0.074	0.034	0.816
Rmeas	0.080	0.037	0.887
Rpim	0.031	0.014	0.342
Total number of observations	944905
Number of reflections	157977
<I/σ(I)>	9.9
Completeness [%]	94.3	99.1	91.6
Redundancy	6	7.1	6.1
CC(1/2)		0.999	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	15% PEG 8000, 0.1M TRIS-HCl, 0.07M K2SO4,