4XEO
Crystal Structure of human AlaRS catalytic domain with R329H mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2011-11-25 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.848, 121.172, 68.012 |
| Unit cell angles | 90.00, 118.47, 90.00 |
Refinement procedure
| Resolution | 33.960 - 1.380 |
| R-factor | 0.1841 |
| Rwork | 0.183 |
| R-free | 0.21390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hxu |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.118 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.400 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.403 | |
| Number of reflections | 185858 | |
| <I/σ(I)> | 29 | 2.6 |
| Completeness [%] | 98.3 | 82.9 |
| Redundancy | 3.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | PEG3350 |
