4XEN
High pressure protein crystallography of hen egg white lysozyme in complex with Tetra-N-acetylchitotetraose at 920 MPa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2014-12-10 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.75 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 76.152, 76.152, 37.962 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.110 - 1.550 |
| R-factor | 0.16686 |
| Rwork | 0.165 |
| R-free | 0.20969 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lzc |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.165 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.286 | |
| Number of reflections | 16785 | |
| <I/σ(I)> | 44.5 | 9.59 |
| Completeness [%] | 99.3 | 98.9 |
| Redundancy | 7.4 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 293 | Sodium chloride |
