4XEI
Orthorhombic isomorph of bovine Arp2/3 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.574, 156.981, 178.087 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.408 - 3.874 |
R-factor | 0.2815 |
Rwork | 0.279 |
R-free | 0.32850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k8k |
RMSD bond length | 0.007 |
RMSD bond angle | 1.510 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.874 |
Rmerge | 0.194 |
Number of reflections | 26925 |
<I/σ(I)> | 6.8 |
Completeness [%] | 97.6 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277 | Ethanol 10%, 1,4-butanediol 15%, 100 mM Tris 7.4 |